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Publications:  Prof Richard Pickersgill

Lee MJ, Mantell J, Brown IR et al.(2018). De novo targeting to the cytoplasmic and luminal side of bacterial microcompartments. Nat Commun vol. 9, (1)
Pluta P, Roversi P, Bernardo-Seisdedos G et al.(2018). Structural basis of pyrrole polymerization in human porphobilinogen deaminase. Biochim Biophys Acta
Uddin I, Frank S, Warren MJ et al.(2018). A Generic Self-Assembly Process in Microcompartments and Synthetic Protein Nanotubes. Small vol. 14, (19)
Abbott W, Alber O, Bayer E et al.(2017). Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes. GLYCOBIOLOGY vol. 28, (1) 3-8.
Gu S, Vladimir VE, Shaw R et al.(2017). The role of intrinsic disorder and dynamics in the assembly and function of the type II secretion system. BBA - Proteins and Proteomics
Qui J, Zeng Y, Pickersgill R et al. (2017). Introducing quasi-optical terahertz circular dichroism spectroscopy. 2017 11th International Conference on Antenna Theory and Techniques, ICATT 2017. 26-29.
Gu S, Sushko O, Deery E et al.(2015). Crystal structure of CobK reveals strand-swapping between Rossmann-fold domains and molecular basis of the reduced precorrin product trap. Sci Rep vol. 5,
Sushko O, Qiu J, Dubrovka R et al. (2015). Sub-terahertz spectroscopy as a probe for protein stability in an ionic environment. IRMMW-THz 2015 - 40th International Conference on Infrared, Millimeter, and Terahertz Waves.
Ali S, Søndergaard CR, Teixeira S et al.(2015). Structural insights into the loss of catalytic competence in pectate lyase activity at low pH. FEBS Lett vol. 589, (21) 3242-3246.
Pang A, Frank S, Brown I et al.(2014). Structural insights into higher order assembly and function of the bacterial microcompartment protein PduA. J Biol Chem vol. 289, (32) 22377-22384.
Lawrence AD, Taylor SL, Scott A et al.(2014). FAD binding, cobinamide binding and active site communication in the corrin reductase (CobR). Biosci Rep vol. 34, (4)
Palmer DJ, Schroeder S, Lawrence AD et al.(2014). The structure, function and properties of sirohaem decarboxylase - an enzyme with structural homology to a transcription factor family that is part of the alternative haem biosynthesis pathway. MOLECULAR MICROBIOLOGY vol. 93, (2) 247-261.
Terali K, Beavil RL, Pickersgill RW et al.(2013). The effect of the adaptor protein Isd11 on the quaternary structure of the eukaryotic cysteine desulphurase Nfs1. Biochem Biophys Res Commun vol. 440, (2) 235-240.
Rehman S, Gu S, Shevchik VE et al.(2013). Anatomy of secretin binding to the Dickeya dadantii type II secretion system pilotin. Acta Crystallogr D Biol Crystallogr vol. 69, (Pt 8) 1381-1386.
DONNAN RS, Pickersgill R, Krauss N et al.(2013). Lagrangian dynamics analysis of sub-THz skeletal absorption spectra of amino acids acquired by vector network analysis. Chemical Physics Letters vol. 558, 104-108.
Pang A, Liang M, Prentice MB et al.(2012). Substrate channels revealed in the trimeric Lactobacillus reuteri bacterial microcompartment shell protein PduB. Acta Crystallogr D Biol Crystallogr vol. 68, (Pt 12) 1642-1652.
Deery E, Schroeder S, Lawrence AD et al.(2012). An enzyme-trap approach allows isolation of intermediates in cobalamin biosynthesis. NATURE CHEMICAL BIOLOGY vol. 8, (11) 933-940.
Wang X, Pineau C, Gu S et al.(2012). Cysteine Scanning Mutagenesis and Disulfide Mapping Analysis of Arrangement of GspC and GspD Protomers within the Type 2 Secretion System. JOURNAL OF BIOLOGICAL CHEMISTRY vol. 287, (23) 19082-19093.
Gu S, Kelly G, Wang X et al.(2012). Solution structure of homology region (HR) domain of type II secretion system. J Biol Chem vol. 287, (12) 9072-9080.
Martin JE, Chowdhury ATMD, McElwaine S et al. (2012). Intestinal Glycogen Storage Disease, and the Molecular Basis of Pseudo-obstruction with Polyglucosan Bodies. JOURNAL OF PATHOLOGY. vol. 226, S24-S24.
Pickersgill RW (2012). Pathology: A Structural Biologist's View. JOURNAL OF PATHOLOGY. vol. 226, S7-S7.
Gu S, Rehman S, Wang X et al.(2012). Structural and functional insights into the pilotin-secretin complex of the type II secretion system. PLoS Pathog vol. 8, (2)
Martin JE, Chowdhury AH, McElwaine S et al. (2011). A Novel Phenotype of Glycogen Storage Disease Presenting as Gastrointestinal Neuromuscular Disease. GASTROENTEROLOGY. vol. 140, S705-S705.
Pang A, Warren MJ, Pickersgill RW(2011). Structure of PduT, a trimeric bacterial microcompartment protein with a 4Fe-4S cluster-binding site. Acta Crystallogr D Biol Crystallogr vol. 67, (Pt 2) 91-96.
Romao CV, Ladakis D, Lobo SAL et al.(2011). Evolution in a family of chelatases facilitated by the introduction of active site asymmetry and protein oligomerization. P NATL ACAD SCI USA vol. 108, (1) 97-102.
Brocklehurst K, Gul S, Pickersgill RW(2010). Substrate Recognition. Amino Acids, Peptides and Proteins in Organic Chemistry, vol. 2,
Seyedarabi A, Hutchison T, To TT et al.(2010). Cloning, purification and preliminary crystallographic analysis of cobalamin methyltransferases from Rhodobacter capsulatus. Acta Crystallogr Sect F Struct Biol Cryst Commun vol. 66, (Pt 12) 1652-1656.
Seyedarabi A, Sullivan JA, Sasakawa C et al.(2010). A disulfide driven domain swap switches off the activity of Shigella IpaH9.8 E3 ligase. FEBS Lett vol. 584, (19) 4163-4168.
Shala K, Yang B, TT T et al. (2010). Millimeter wave spectroscopy of glucose and a calcium-binding protein using a vector network analyser. Conference: 35th IRMMW-THZ Internation Conference, 2010 (Rome, Italy) from: 05/09/2010 to: 10/09/2010, 1-2.
Login FH, Fries M, Wang XH et al.(2010). A 20-residue peptide of the inner membrane protein OutC mediates interaction with two distinct sites of the outer membrane secretin OutD and is essential for the functional type II secretion system in Erwinia chrysanthemi. Molecular Microbiology vol. 76, (4) 944-955.
Seyedarabi A, To TT, Ali S et al.(2010). Structural insights into substrate specificity and the anti beta-elimination mechanism of pectate lyase. Biochemistry vol. 49, (3) 539-546.
Heldt D, Frank S, Seyedarabi A et al.(2009). Structure of a trimeric bacterial microcompartment shell protein, EtuB, associated with ethanol utilization in Clostridium kluyveri. BIOCHEM J vol. 423, 199-207.
Brindley AA, Pickersgill RW, Partridge JC et al.(2008). Enzyme sequence and its relationship to hyperbaric stability of artificial and natural fish lactate dehydrogenases. PLoS One vol. 3, (4)
Lawrence AD, Deery E, McLean KJ et al.(2008). Identification, characterization, and structure/function analysis of a corrin reductase involved in adenosylcobalamin biosynthesis. J BIOL CHEM vol. 283, (16) 10813-10821.
Fries M, Ihrig J, Brocklehurst K et al.(2007). Molecular basis of the activity of the phytopathogen pectin methylesterase. EMBO J vol. 26, (17) 3879-3887.
Frank S, Deery E, Brindley AA et al.(2007). Elucidation of substrate specificity in the cobalamin (vitamin B12) biosynthetic methyltransferases. Structure and function of the C20 methyltransferase (CbiL) from Methanothermobacter thermautotrophicus. J Biol Chem vol. 282, (33) 23957-23969.
Ooi A, Hussain S, Seyedarabi A et al.(2006). Structure of internalin C from Listeria monocytogenes. Acta Crystallogr D Biol Crystallogr vol. 62, (Pt 11) 1287-1293.
Opaleye O, Rose R-S, Whittaker MM et al.(2006). Structural and spectroscopic studies shed light on the mechanism of oxalate oxidase. J Biol Chem vol. 281, (10) 6428-6433.
Graham CE, Brocklehurst K, Pickersgill RW et al.(2006). Characterization of retinaldehyde dehydrogenase 3. Biochem J vol. 394, (Pt 1) 67-75.
Frank S, Brindley AA, Deery E et al.(2005). Anaerobic synthesis of vitamin B12: characterization of the early steps in the pathway. Biochem Soc Trans vol. 33, (Pt 4) 811-814.
Okuda J, Toyotome T, Kataoka N et al.(2005). Shigella effector IpaH9.8 binds to a splicing factor U2AF(35) to modulate host immune responses. BIOCHEM BIOPH RES CO vol. 333, (2) 531-539.
Jenkins J, Shevchik VE, Hugouvieux-Cotte-Pattat N et al.(2004). The crystal structure of pectate lyase Pel9A from Erwinia chrysanthemi. J BIOL CHEM vol. 279, (10) 9139-9145.
Pickersgill RW(2003). A primordial structure underlying amyloid. STRUCTURE vol. 11, (2) 137-138.
PICKERSGILL RW, Jenkins JA (2003). The structures and active sites of pectinases. Advances in pectin and pectinase research. Editors: Voragen, AGJ, Voragen, F, Schols, H, Visser, R et al., Conference: International symposium on pectins and pectinases (2nd) (Rotterdam, The Netherlands) from: 05/2001 to: 05/2001, 267-278.
Pickersgill RW(2002). Complex cell signaling molecules from ancient molecular glue. STRUCTURE vol. 10, (10) 1287-1288.
Woo EJ, Marshall J, Bauly J et al.(2002). Crystal structure of auxin-binding protein 1 in complex with auxin. EMBO J vol. 21, (12) 2877-2885.
Palomäki T, Pickersgill R, Riekki R et al.(2002). A putative three-dimensional targeting motif of polygalacturonase (PehA), a protein secreted through the type II (GSP) pathway in Erwinia carotovora. Mol Microbiol vol. 43, (3) 585-596.
Lo Leggio L, Kalogiannis S, Eckert K et al.(2001). Substrate specificity and subsite mobility in T. aurantiacus xylanase 10A. FEBS Lett vol. 509, (2) 303-308.
Jenkins J, Pickersgill R(2001). The architecture of parallel beta-helices and related folds. PROG BIOPHYS MOL BIO vol. 77, (2) 111-175.
Hogg D, Woo EJ, Bolam DN et al.(2001). Crystal structure of mannanase 26A from Pseudomonas cellulosa and analysis of residues involved in substrate binding. J Biol Chem vol. 276, (33) 31186-31192.
Teixeira S, Lo Leggio L, Pickersgill R et al.(2001). Anisotropic refinement of the structure of Thermoascus aurantiacus xylanase I. ACTA CRYSTALLOGR D vol. 57, 385-392.
Jenkins J, Mayans O, Smith D et al.(2001). Three-dimensional structure of Erwinia chrysanthemi pectin methylesterase reveals a novel esterase active site. J Mol Biol vol. 305, (4) 951-960.
Leggio LL, Jenkins J, Harris GW et al.(2000). X-ray crystallographic study of xylopentaose binding to Pseudomonas fluorescens xylanase A. Proteins vol. 41, (3) 362-373.
Woo EJ, Bauly J, Chen JG et al.(2000). Crystallization and preliminary X-ray analysis of the auxin receptor ABP1. Acta Crystallogr D Biol Crystallogr vol. 56, (Pt 11) 1476-1478.
Woo EJ, Dunwell JM, Goodenough PW et al.(2000). Germin is a manganese containing homohexamer with oxalate oxidase and superoxide dismutase activities. Nat Struct Biol vol. 7, (11) 1036-1040.
Lo Leggio L, Pickersgill RW(1999). Xylanase-oligosaccharide interactions studied by a competitive enzyme assay. Enzyme and Microbial Technology vol. 25, (8-9) 701-709.
Lo Leggio L, Kalogiannis S, Bhat MK et al.(1999). High resolution structure and sequence of T. aurantiacus xylanase I: implications for the evolution of thermostability in family 10 xylanases and enzymes with (beta)alpha-barrel architecture. Proteins vol. 36, (3) 295-306.
Scott M, Pickersgill RW, Hazlewood GP et al.(1999). Crystallization and preliminary X-ray analysis of arabinanase A from Pseudomonas fluorescens subspecies cellulosa. Acta Crystallogr D Biol Crystallogr vol. 55, (Pt 2) 544-546.
Pickersgill R, Scott M, Smith D et al.(1999). Crystallization and preliminary crystallographic analysis of the endo-polygalacturonase from Erwinia carotovora ssp. carotovora. Acta Crystallogr D Biol Crystallogr vol. 55, (Pt 1) 320-322.
Aliwan FO, Kroon PA, Faulds CB et al.(1999). Ferulic acid esterase-III from Aspergillus niger does not exhibit lipase activity. Journal of the Science of Food and Agriculture vol. 79, (3) 457-459.
Faulds CB, Aliwan FO, Vries RPD et al.(1998). Chemical and thermal stability of ferulic acid (feruloyl) esterases from Aspergillus. Progress in Biotechnology vol. 15, (C) 41-46.
Woo EJ, Dunwell JM, Goodenough PW et al.(1998). Barley oxalate oxidase is a hexameric protein related to seed storage proteins: evidence from X-ray crystallography. FEBS Lett vol. 437, (1-2) 87-90.
Pickersgill R, Smith D, Worboys K et al.(1998). Crystal structure of polygalacturonase from Erwinia carotovora ssp. carotovora. J Biol Chem vol. 273, (38) 24660-24664.
Pickersgill R, Harris G, Lo Leggio L et al. (1998). Superfamilies: the 4/7 superfamily of beta alpha-barrel glycosidases and the right-handed parallel beta-helix superfamily. Biochem Soc Trans. vol. 26, 190-198.
Scott M, Pickersgill RW, Hazlewood GP et al.(1998). Crystallization and preliminary X-ray diffraction studies of a family 26 endo-beta-1,4 mannanase (ManA) from Pseudomonas fluorescens subspecies cellulosa. Acta Crystallogr D Biol Crystallogr vol. 54, (Pt 1) 129-131.
Jenkins J, Mayans O, Pickersgill R(1998). Structure and evolution of parallel beta-helix proteins. J Struct Biol vol. 122, (1-2) 236-246.
Harris GW, Pickersgill RW, Connerton I et al.(1997). Structural basis of the properties of an industrially relevant thermophilic xylanase. Proteins vol. 29, (1) 77-86.
Spurway TD, Morland C, Cooper A et al.(1997). Calcium protects a mesophilic xylanase from proteinase inactivation and thermal unfolding. J Biol Chem vol. 272, (28) 17523-17530.
Mayans O, Scott M, Connerton I et al.(1997). Two crystal structures of pectin lyase A from Aspergillus reveal a pH driven conformational change and striking divergence in the substrate-binding clefts of pectin and pectate lyases. Structure vol. 5, (5) 677-689.
Charnock SJ, Lakey JH, Virden R et al.(1997). Key residues in subsite F play a critical role in the activity of Pseudomonas fluorescens subspecies cellulosa xylanase A against xylooligosaccharides but not against highly polymeric substrates such as xylan. J Biol Chem vol. 272, (5) 2942-2951.
Lo Leggio L, Parry NJ, Van Beeumen J et al.(1997). Crystallization and preliminary X-ray analysis of the major endoglucanase from Thermoascus auranticus. Acta Crystallographica Section D: Biological Crystallography vol. 53, (5) 599-604.
Groves MR, Taylor MA, Scott M et al.(1996). The prosequence of procaricain forms an alpha-helical domain that prevents access to the substrate-binding cleft. Structure vol. 4, (10) 1193-1203.
Katerelos NA, Taylor MA, Scott M et al.(1996). Crystal structure of a caricain D158E mutant in complex with E-64. FEBS Lett vol. 392, (1) 35-39.
Jenkins J, Scott M, Mayans O et al.(1996). Crystallization and preliminary X-ray analysis of pectin lyase a from Aspergillus niger. Acta Crystallographica Section D: Biological Crystallography vol. 52, (2) 402-404.
Harris GW, Jenkins JA, Connerton I et al.(1996). Refined crystal structure of the catalytic domain of xylanase a from Pseudomonas fluorescens at 1.8 Å resolution. Acta Crystallographica Section D: Biological Crystallography vol. 52, (2) 393-401.
Jenkins J, Lo Leggio L, Harris G et al.(1995). Beta-glucosidase, beta-galactosidase, family A cellulases, family F xylanases and two barley glycanases form a superfamily of enzymes with 8-fold beta/alpha architecture and with two conserved glutamates near the carboxy-terminal ends of beta-strands four and seven. FEBS Lett vol. 362, (3) 281-285.
Smyth MG, Pickersgill RW, Lax AJ(1995). The potent mitogen Pasteurella multocida toxin is highly resistant to proteolysis but becomes susceptible at lysosomal pH. FEBS Lett vol. 360, (1) 62-66.
Harris GW, Jenkins JA, Connerton I et al.(1994). Structure of the catalytic core of the family F xylanase from Pseudomonas fluorescens and identification of the xylopentaose-binding sites. Structure vol. 2, (11) 1107-1116.
Taylor MA, Baker KC, Connerton IF et al.(1994). An unequivocal example of cysteine proteinase activity affected by multiple electrostatic interactions. Protein Eng vol. 7, (10) 1267-1276.
Pickersgill R, Jenkins J, Harris G et al.(1994). The structure of Bacillus subtilis pectate lyase in complex with calcium. Nat Struct Biol vol. 1, (10) 717-723.
Pickersgill R, Varvill K, Jones S et al.(1994). Making a small enzyme smaller; removing the conserved loop structure of hen lysozyme. FEBS Lett vol. 347, (2-3) 199-202.
Loris R, Steyaert J, Maes D et al.(1993). Crystal structure determination and refinement at 2.3-A resolution of the lentil lectin. Biochemistry vol. 32, (51)
Loris R, Steyaert J, Maes D et al.(1993). Erratum: Crystal structure determination and refinement at 2.3-Å resolution of the lentil lectin (Biochemistry (1993) 32, 34, (8772-8781)). Biochemistry vol. 32, (51)
Bhat MK, Pickersgill RW, Perry BN et al.(1993). Modification of the head-group selectivity of porcine pancreatic phospholipase A2 by protein engineering. Biochemistry vol. 32, (45) 12203-12208.
Loris R, Steyaert J, Maes D et al.(1993). Crystal structure determination and refinement at 2.3-A resolution of the lentil lectin. Biochemistry vol. 32, (34) 8772-8781.
Sumner IG, Vaughan A, Eisenthal R et al.(1993). Kinetic analysis of papaya proteinase omega. Biochim Biophys Acta vol. 1164, (3) 243-251.
Revell DF, Cummings NJ, Baker KC et al.(1993). Nucleotide sequence and expression in Escherichia coli of cDNAs encoding papaya proteinase omega from Carica papaya. Gene vol. 127, (2) 221-225.
Sumner IG, Harris GW, Taylor MA et al.(1993). Factors effecting the thermostability of cysteine proteinases from Carica papaya. Eur J Biochem vol. 214, (1) 129-134.
Pickersgill RW, Debeire P, Debeire-Gosselin M et al.(1993). Crystallization and preliminary X-ray analysis of a thermophilic Bacillus xylanase. Journal of Molecular Biology vol. 230, (2) 664-666.
Golubev AM, Kilimnik AYu, Neustroev KN et al.(1993). Crystals of beta-xylanase from Aspergillus oryzae. J Mol Biol vol. 230, (2) 661-663.
Jones ST, Ahlström P, Berendsen HJ et al.(1993). Molecular dynamics simulation of a phospholipase A2-substrate complex. Biochim Biophys Acta vol. 1162, (1-2) 135-142.
Pickersgill RW, Jenkins JA, Scott M et al.(1993). Crystallization and preliminary X-ray analysis of the catalytic domain of xylanase A from Pseudomonas fluorescens subspecies cellulosa. Journal of Molecular Biology vol. 229, (1) 246-248.
Pickersgill RW(1993). Design and engineering of hydrolytic enzymes for enhanced performance in the agricultural and food industries. Journal of Chemical Technology &amp; Biotechnology vol. 57, (3) 290-291.
Jenkins J, Nasser W, Scott M et al.(1992). Crystallization and preliminary X-ray studies of the pectate lyase from Bacillus subtilis. J Mol Biol vol. 228, (4) 1255-1258.
Harris GW, Pickersgill RW, Howlin B et al.(1992). The segmented anisotropic refinement of monoclinic papain by the application of the rigid-body TLS model and comparison to bovine ribonuclease A. Acta Crystallogr B vol. 48 ( Pt 1), 67-75.
Loris R, Lisgarten J, Maes D et al.(1992). Two crystal forms of the lentil lectin diffract to high resolution. J Mol Biol vol. 223, (2) 579-581.
Pickersgill RW, Harris GW, Garman E(1992). Structure of monoclinic papain at 1.60 Å resolution. Acta Crystallographica Section B vol. 48, (1) 59-67.
Goodenough PW, Bhat KM, Collins ME et al.(1991). Changes in activity of porcine phospholipase A2 brought about by charge engineering of a major structural element to alter stability. Protein Eng vol. 4, (8) 929-934.
Bhat KM, Sumner IG, Perry BN et al.(1991). A novel method for the purification of porcine phospholipase A2 expressed in E. coli. Biochem Biophys Res Commun vol. 176, (1) 371-377.
Pickersgill RW, Sumner IG, Collins ME et al.(1991). Modification of the stability of phospholipase A2 by charge engineering. FEBS Lett vol. 281, (1-2) 219-222.
Pickersgill RW, Rizkallah P, Harris GW et al.(1991). Determination of the structure of papaya protease omega. Acta Crystallographica Section B vol. 47, (5) 766-771.
Pickersgill RW, Goodenough PW(1991). Enzyme engineering. Trends in Food Science and Technology vol. 2, (C) 122-126.
Pickersgill RW, Sumner IG, Goodenough PW(1990). Preliminary crystallographic data for protease omega. Eur J Biochem vol. 190, (2) 443-444.
Pickersgill RW, Sumner IG, Collins ME et al.(1989). Structural and electrostatic differences between actinidin and papain account for differences in activity. Biochem J vol. 257, (1) 310-312.
Pickersgill RW(1988). A rapid method of calculating charge-charge interaction energies in proteins. Protein Eng vol. 2, (3) 247-248.
Pickersgill RW, Goodenough PW, Sumner IG et al.(1988). The electrostatic fields in the active-site clefts of actinidin and papain. Biochem J vol. 254, (1) 235-238.
Pickersgill RW(1987). One‐dimensional disorder in spinach ribulose bisphosphate carboxylase crystals. Acta Crystallographica Section A vol. 43, (4) 502-506.
Adams MJ, Gover S, Pickersgill RW et al.(1983). Structure--function relationships of NAD+- and NADP+-dependent dehydrogenases with particular reference to the three-dimensional structure of 6-phosphogluconate dehydrogenase. Biochem Soc Trans vol. 11, (4) 429-435.
Adams MJ, Archibald IG, Bugg CE et al.(1983). The three dimensional structure of sheep liver 6-phosphogluconate dehydrogenase at 2.6 A resolution. EMBO J vol. 2, (6) 1009-1014.
GARNETT JA, Gu, S, Shevchik VE et al.. The role of intrinsic disorder and dynamics in the assembly and function of the type II secretion system. BBA - Proteins and Proteomics
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